Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From
Two-Dimensional and Three-Dimensional Cell Culture Microenvironments
Abstract
Bone marrow derived stem cells express biomarkers capable of
facilitating adhesion to the cell culturing microenvironment, thereby,
influencing their proliferation, migration, and differentiation. In
particular, biological biomarkers of mesenchymal stem cells include, but
are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+,
CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between
the stem cell biology and the materials and methods forming a cell
culturing microenvironment serves as a critical aspect in the successful
adhesion and growth within two-dimensional cell culture
microenvironments such as polystyrene, laminin, fibronectin, or
poly-L-lysine and within three-dimensional cell culture
microenvironments such as hydrogel, ceramic, collagen, polymer based
nanofibers, agitation, forced floating, and hang drop systems. Further,
electrical stimulation of the stem cells may be implemented during the
cell culturing process to measure stem cell growth and to determine stem
cell viability. In addition, electrical stimulation of implanted stem
cells may facilitate tracking by measuring stem cell migration distance
and travel area. Although many biochemical and inflammatory biomarkers
are expressed based on severity in stroke including, but not limited to,
Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate
(Glu), current methodologies of stem cell directing lack localization
and biological effector specificity. Biological effector bound magnetic
particle stem cells may serve as a potential treatment method in
ischemic stroke. In particular, a stem cell biomarker may be configured
to communicate with inflammatory biomarkers, thus, more efficiently
delivering the stem cells to site specific areas having the most
severely affected in-vivo biochemical microenvironments.