Towards Ultraviolet Microbeam Scanning and Lens-Less UV Microbeam
Microscopy with Mirror Galvanometric Scanners: From the History of
Research Instrumentation to Engineering of Modern Mechatronic Optical
Systems
Abstract
This article aims to ensure continuity between classical methods of
ultraviolet microscopy or/and micromanipulation using ultraviolet
microbeam and lens-less ultraviolet microscopy and microbeam exposure of
cells and tissues. Considering the history of the development of the
method and the possibility of working with different methods of
mechanical scanning, the authors propose to use mirror galvanometers and
an electromechanical scanning system in the mechanical engineering of
lensless microbeam installations. These installations make it possible
to provide both scanning with an ultraviolet microbeam to obtain a line
scan image, and precision micromanipulation at the level of individual
cells. This article is a preprint (or reprint) associated with the
authors’ presentation at the September 2018 seminar dedicated to the
memory of Sergei Chakhotin, the developer of the first UV microbeam
installations. The deposit of this article in the technical archive in
September of this year is associated with a memorable date associated
with the dates of the life of Sergei Chakhotin (13 September 1883 – 24
December 1973). The first article by one of the authors, which formed
the basis for this presentation, was also timed to coincide with the
memorable date of Sergei Chakhotin and was presented at the Christmas
seminar at the end of 2013 (December 24), and was published in an issue
of the Ukrainian bioengineering journal in 2014.