Gene categories with differential regulatory element activity
To obtain information about potential pathways affected by the treatment
(host plant availability), we identified differentially acetylated or
methylated regions between the treatment groups. For the H3K27ac mark,
we identified 9 proximal gene regions with significant differential
activity peaks between treatment groups (Figure 2). Seven of the genes
in the vicinity of H3K27ac peaks with differential activity had
annotation information, suggesting that they are involved in regulation
of female receptivity to mating (1 gene), chorion (insect egg shell)
gene amplification (1 gene), pheromone binding activity / sensory
perception of smell (1 gene), actin binding in muscle tissue and motor
neuron to muscle communication (2 genes), regulation of juvenile hormone
(1 gene) and mini-chromosome maintenance (1 gene) (Supplementary Table
1). For the H3K4me3 mark, there were no significantly differentially
activated regions after adjusting for multiple testing.