DNA extractions
The DNA was extracted using FastDNAâ„¢ SPIN Kit for Soil (MP). For the qPCR, the 1:10 and 1:100 dilutions of the samples were used for extraction. The quality and quantity of the extractions was confirmed by 1% agarose gel electrophoresis and dsDNA BR (Qubit) respectively. The extracted DNA was used later to determine the cell densities using qPCR and for sequencing.