Measuring methane content of biogas
All resulting lab-scale reactors inoculated with the samples were
monitored for six weeks after the first feeding at 37°C using an AMPTS
II (BPC Instruments) system that measures the volume of biogas stripped
from CO2 using sodium hydroxide. We confirmed that the
resulting stripped biogas was >95% methane by comparing
the composition of the produced biogas pre- and post-stripping using
GC-MS (Agilent, 7890A) and comparing these with a standard curve made
using methane standard (Sigma).