Measuring methane content of biogas
All resulting lab-scale reactors inoculated with the samples were monitored for six weeks after the first feeding at 37°C using an AMPTS II (BPC Instruments) system that measures the volume of biogas stripped from CO2 using sodium hydroxide. We confirmed that the resulting stripped biogas was >95% methane by comparing the composition of the produced biogas pre- and post-stripping using GC-MS (Agilent, 7890A) and comparing these with a standard curve made using methane standard (Sigma).