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Single-cell sequencing reveals the immune landscape of breast cancer patients with brain metastasis
  • Huaping Zhou
Huaping Zhou
Affiliated Cancer Hospital & Institute of Guangzhou Medical University

Corresponding Author:[email protected]

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Abstract

Breast cancer, cancer with the highest incidence rate worldwide, is also the most common cause of cancer death for women in all countries. Among them, brain metastasis (BrM) is a characteristic of a severely poor prognosis for breast cancer. Due to the unique anatomy of brain metastases, the understanding of the tumor immune microenvironment (TME) of BrM from breast cancer is limited, which hinders our diagnosis and treatment of breast cancer patients.
In this study, we used single-cell RNA sequencing (scRNA-seq) to characterize immune cells in the cerebrospinal fluid (CSF) of BrM patients to describe the immune landscape of BrM. We identified 9 cell subtypes: T cells, Tregs, MonoMacs, Macs, TAMs, Myeloid cells, DCs, Endo_Fibs and Glioblastals from CSF samples and analyzed their functions in the TME. In addition, we classified T cell, macrophage and DC subclusters by UMAP analysis and compared the differential genes and biological processes of each subcluster with changes in prognosis. Finally, we also analyzed cell-cell interactions among cell subclusters and their differences.
As our research focuses on the tumor immune microenvironment of brain metastasis, we believe that our research will be attractive not only to cancer immunologists but also to the interdisciplinary readership of Cancer Science.